Scalable Lentiviral Vector Production Using Stable HEK293SF Producer Cell Lines
نویسندگان
چکیده
منابع مشابه
Scalable Lentiviral Vector Production Using Stable HEK293SF Producer Cell Lines
Lentiviral vectors (LV) represent a key tool for gene and cell therapy applications. The production of these vectors in sufficient quantities for clinical applications remains a hurdle, prompting the field toward developing suspension processes that are conducive to large-scale production. This study describes a LV production strategy using a stable inducible producer cell line. The HEK293 cell...
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Lentiviral vectors are useful experimental tools for stable gene delivery and have been used to treat human inherited genetic disorders and hematologic malignancies with promising results. Because some of the lentiviral vector components are cytotoxic, transient plasmid transfection has been used to produce the large batches needed for clinical trials. However, this method is costly, poorly rep...
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The mammalian cell lines HEK293 and CHO have become important expression hosts in structural biology. Generating stable mammalian cell lines remains essential for studying the function and structure of recombinant proteins, despite the emergence of highly efficient transient transfection protocols. Production with stable cell lines can be scaled up easily and high volumetric product yield can b...
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ژورنال
عنوان ژورنال: Human Gene Therapy Methods
سال: 2017
ISSN: 1946-6536,1946-6544
DOI: 10.1089/hgtb.2017.086